Generator

Part:BBa_K2315018

Designed by: FANG BA   Group: iGEM17_Shanghaitech   (2017-10-21)

The HSL receiver TraR from Rhizobium radiobacter activates expression of GFP protein in response to 3OC8-HSL.

This Receiver can be easily replaced by other AHL receivers in our collection. A full collection could be found in: http://2017.igem.org/Team:Shanghaitech/Library

Usage and Biology

Fig. 1 Genetic Circuit Design

When AHL is added with concentration higher than a critical value, the constitutively expressed TraR will bind to the AHL molecule 3OC8-HSL, dimerize and bind to the pTra regulatory sequence to activate GFP expression.

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